Hoopes was born in Kuala Lumpur, Malaysia and was the fourth of five children in her family. She spent her childhood travelling overseas, particularly to America, where she received her Bachelor of Fine Arts degree at New York University's Tisch School of the Arts. Her first major acting accomplishment was her creation of the character Bee Bee in Eric Bogosian's production SubUrbia at Lincoln Street. Her next notable achievement was receiving multiple roles on Daria.
Since Daria ended in 2002, Hoopes has made guest appearances on many television shows, including Law & Order: Criminal Intent, Hey Joel, The Wrong Coast, The Jury, LAX, Judging Amy, Third Watch, Sex and the City, Law & Order, Swift Justice, and New York Undercover. Her film credits include Private Parts, Better Living, Killing Cinderella, Calling Bobcat, Roadblock and Spinster.
She has also featured in many theatre productions, include a rock musical about Janis Joplin, and she provided the voice for Mona Sax in the video game Max Payne 2: The Fall of Max Payne.
Ms. Hoopes's regular speaking voice is closest to Jane Lane's.